School of Biosecurity, Biotechnolgy and Laboratory Sciences (SBLS) Collection
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ItemAntibiotic susceptibility and selected toxin genes profiles among Bacillus cereus isolated from raw bovine milk from a milk collection center in Kawempe Division, Kampala District(Makerere University, 2023)Bacillus cereus causes food intoxication hence a burden to public health. Bacillus cereus is also among the top five foodborne pathogens, especially in starchy foods and raw cow milk. The pathogenicity of the B. cereus is associated with the potential to produce diarrheal and emetic toxins. The pathogen has been associated with clinical infections, whose management could be challenged by antimicrobial resistance. There is scanty information on the contamination levels of raw bovine milk with B. cereus, its toxigenic potential, and its susceptibility to antibiotics in Uganda. This study aimed to determine the proportion of raw bovine milk contaminated with B. cereus sold within Kawempe division, Kampala as well as its toxigenic potential and antibiotic susceptibility profiles. In the present study, 30 raw bovine milk samples were collected from a milk collection centre in the Kawempe division, Kampala . From the samples where growth occurred, five isolates were selected for further characterization. Isolates were identified and confirmed using gram-staining, citrate, catalase, oxidase and motility. Antibiotic susceptibility profiles were determined by the Kirby-Bauer technique, while multiplex real-time PCR allowed the detection of toxigenic genes (hblD, nheA, cytK 1 and ces). Of the 30 milk samples, eight (26.7%) were contaminated with B. cereus. All the 40 isolates that were selected for antibiotic sensitivity testing, exhibited susceptibility to gentamycin, tetracycline, chloramphenicol and ciprofloxacin, but all were resistant to ß-lactam products including Ampicillin and Penicillin. The hblD, nheA, cytK1 and ces were encountered in 82.5 % (33/40), 85 % (34/40), 0%, and 0% of the B. cereus isolates respectively. Contamination of milk with B. cereus and the presence of the diarrheal toxin genes, hblD and nheA, implies that there is a potential risk of associated food-borne illnesses, especially where raw bovine milk is consumed. The cytK1, a diarrheal toxin-encoding gene was absent. In this research, the ces was absent hence no potential for emesis. Thus, there is a need for wider surveillance of contamination of especially the raw bovine milk.
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ItemMathematical modeling and simulation of malaria vector propagation : a tool for evaluation of novel control tools(Makerere University, 2026)Background: Malaria transmission is sustained by highly adaptable Anopheles mosquitoes that persist across dry seasons and rebound in large numbers when the rains return. Despite major progress in vector control, knowledge gaps remain regarding the survival mechanisms that sustain mosquito populations during dry seasons. This study investigated the evolutionary and ecological mechanisms enabling such persistence and spread. It contributes critical insights for evaluating the effectiveness and sustainability of current and emerging vector control tools, including gene drive technologies. Methods: To investigate the dry season persistence mechanisms of malaria mosquitoes, existing studies on survival strategies were critically evaluated to identify strengths, weaknesses, and knowledge gaps. A novel population genetic modeling framework was developed to estimate the proportion of aestivating adults, mosquitoes typically difficult to sample due to unknown habitats, and was initially applied to the Anopheles coluzzii dataset from Mali. This model was subsequently extended to jointly estimate both aestivation and long-distance migration and then applied to temporal genetic data from Eastern Uganda. Additionally, to assess the potential impact of novel vector control strategies such as gene drive, the population genetic structure and demographic history of Anopheles gambiae and Anopheles arabiensis were characterized using amplicon sequencing data collected from three island and three mainland sites in Uganda. Results: Malaria mosquito populations persist through the dry season and rapidly rebound at the onset of the rainy season via four key mechanisms: aestivation, local refugia, local migration, and long-distance migration. Application of the developed population genetic model to temporal data from Mali successfully estimated the proportion of aestivating adults. When extended to incorporate both aestivation and long-distance migration and applied to temporal data from Eastern Uganda, the model revealed that the Sahelian region exhibits stronger seasonality compared to Eastern Uganda. Furthermore, genomic analysis of amplicon sequencing data from island and mainland sites in Uganda showed pronounced spatial population structure, with island populations showing greater genetic differentiation not only from mainland populations but also among individual island sites. This strong within-island differentiation highlights their potential suitability for contained gene drive field trials. Conclusion: This study aimed to determine the role of seasonal and evolutionary dynamics in mosquito survival by demonstrating that population genetics models can effectively estimate proportions of aestivating mosquitoes typically difficult to sample in the field. Moreover, this modelling framework is adaptable to quantify the relative contributions of multiple mosquito survival mechanisms within diverse ecological contexts. These insights are critical for optimizing the design of field trials for novel vector control strategies, such as gene drive, by elucidating population connectivity and seasonal persistence patterns. Additionally, the findings contribute to improved insecticide resistance management by illuminating how seasonal dynamics and vector life-history strategies influence the strength of selection and the spread of adaptive alleles, thereby supporting the responsible and sustainable deployment of genetic control technologies.
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ItemHIV drug resistant mutations associated with virological failure among HIV-1 zero positive infants in Uganda after intensified adherence counseling(Makerere University, 2026)Paediatric HIV treatment is setback by poor adherence and emerging drug resistance. This longitudinal cohort study assessed the impact of intensified adherence counselling (IAC) on viral suppression and the development of HIV drug resistance mutations in HIV-positive infants receiving antiretroviral therapy (ART). 100 HIV-positive infants (aged 4–12 months) with unsuppressed viral loads (>1000 copies/mL) were enrolled at the Joint Clinical Research Centre (JCRC), Uganda. Mother-infant pairs received IAC for a period of three months. After three months, re-evaluation was conducted, during which HIV viral load was measured using the Abbott assay and adherence was assessed using the pill-count method. Infants with persistent viremia were subjected to drug resistance testing using next-generation sequencing using a MiSeq from which the resulting FASTA files were downloaded from Hydra and submitted to the Stanford University HIV Drug Resistance Database (Version 9.8). The output, provided as a Comma-Separated Values (CSV) file, included classified mutations, mixtures, corresponding scores, susceptibility status, and other relevant parameters. A tabular summary of these findings was then generated to facilitate interpretation and further analysis. Intensified Adherence Counselling (IAC) in mother–infant pairs prompted better results in viral suppression despite changes in adherence, with viral loads continually decreasing after IAC compared to before IAC. HIV drug resistance mutations showed changes in both NRTI and NNRTI profiles. The common NRTI mutation M184V/I reduced slightly post-IAC (43.5% to 40.2%), while increase in TAM-associated mutations (K219, K70, D67) suggested ongoing selective pressure from thymidine-based regimens. NNRTI resistance showed an overall decline, with major mutations such as K103N and Y181C decreasing significantly after IAC, with the disappearance of variants like E138Q and G190S, confirming improved viral suppression and reduced propagation of resistant quasispecies. Mixture mutation analysis showed reductions in NRTI (M184, K219) and NNRTI (V179, V108, Y181, V106) variants, further indicating suppression of resistant viral populations. Emergence and persistence of mutations such as E138A, K238, P225H reflects ongoing viral suppression under treatment pressure. Overall, IAC contributed to improved viral control and reductions in several resistance-associated variants, though there is a continued need for vigilant resistance monitoring and optimized ART regimens.
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ItemMorphological, molecular and histopathological characterization of fungi isolated from formalin-fixed animal cadavers and possible sources of contamination from anatomy laboratory, COVAB, Makerere University(Makerere University, 2025)Animal cadavers play a crucial role as a fundamental instructional resource in the training of anatomists and veterinary students. While formalin is known for its potent antimicrobial properties, certain fungi continue to grow in the presence of formalin. This study aimed at identifying the fungal species that grow on formalin-fixed animal cadavers and internal tissues of fixed cadavers using morphological molecular and histopathological techniques. In addition, the study identified potential environmental sources of contamination. Finally, this study also identified fungal taxa carrying the adhc and fdh genes associated with formalin degradation. The fungi isolated from formalin-fixed animal cadaver tanks, the environmental air from the store, and the embalming room floor, were characterized using PCR amplification and sequencing of the internal transcribed spacer (ITS) region, β-tubulin (Bt), adhc, and fdh genes, together with morphological characterization. The resulting sequences were analyzed using BLAST, and species identification was confirmed by comparison with representative sequences in GenBank. Formalin fixed tissues were processed in the histopathology laboratory and stained with Gridley’s stain. A total of ten fungal isolates belonging to three genera; Aspergillus, Penicillium, and Scedosporium were identified, with Aspergillus being the predominant genus (8/10). Seven isolates originated from the three formalin-fixed animal cadavers. No fungal growth was detected in the distilled water used to prepare the 10% formalin. Two isolates of A. flavus were obtained from the environmental air (store), and one Penicillium species was isolated from the embalming room floor. β-tubulin gene sequencing confirmed Aspergillus niger (isolate 2) and Aspergillus flavus var. flavus (isolate 9). The fdh gene was detected in two Aspergillus isolates whereas adhc formaldehyde-degrading gene was detected in four Aspergillus isolates. The Penicillium isolate contained both genes. Histopathological examination revealed fungal mycelia of Aspergillus in formalin-fixed tissues without accompanying inflammation, indicating post-fixation invasion. The detection of fungal species capable of degrading formaldehyde presents a potential veterinary public health concern. Similar studies should be carried out in other laboratories that use formalin fixed tissues to determine whether comparable contamination challenges exist.
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ItemThe link between Acaricide usage and Oxytetracycline resistance genes in Anaplasma marginale infected cattle blood from selected farms in Kyenjojo, Kumi and Serere Districts(Makerere University, 2026)The management of Bovine Anaplasmosis relies on vector control and chemotherapy. Following the rise of acaricide resistance in Uganda, there is an increase in TBDs incidence, resulting into over reliance on antimicrobials to treat infected animals. Oxytetracycline, being cheap, effective with minimal adverse effects, is mostly used in treating Anaplasmosis. This study was conducted on selected farms in Kyenjojo, Kumi and Serere districts, where resistance in Boophilus decoloratus had been previously reported. The study aimed at investigating otrA and otrB resistance genes in A. marginale infected cattle and access their association with acaricide usage. DNA was extracted from 316 cattle blood samples and screened for msp4 gene. A. marginale positive samples were screened for otrA and otrB using in house primers, and representative samples sequenced using Oxford Nanopore to confirm the PCR product. Six out of the Eight farmers (6/8) in Kyenjojo reported to have used more than one acaricide in the last six months. The proportion of A. marginale was 70% (140/200) and 35.3% (41/116) in Kyenjojo and Kumi/Serere farms respectively. The difference in the frequency of A. marginale between Kyenjojo and Kumi/Serere was statistically significant (P=<0.001). The general proportion of otrA and otrB among the 181 A. marginale infected cattle was 71.8% (130/180) and 47.8% (86/180) respectively. Among Kyenjojo sampled animals, otrA and otrB proportion was 68.6% and 44.3% respectively, while in Kumi & Serere animals, otrA and otrB proportion was 82.9% and 58.5% respectively. Multivariate regression revealed that Exotic breeds were 1.89 times more likely to have otrA compared to local breeds (OR=1.89, 95%CI=0.85-4.40, p=0.13). No acaricide usage factor had a significant relationship with otrA, despite the higher proportions of otrA observed among animals from farms that rotated acaricides due to presence of a new product on market (77.3%), randomly changed acaricides on observing resistance (75.2%) and those that sprayed every week (71.8%). Sequence analysis revealed a 50-55% similarity with sequences available in the database, with study sequences clustering together. This study reveals high proportions of OTC resistance genes in A. marginale infected cattle blood. Animals from farms with poor acaricide use practices had higher proportions of otrA. Larger studies involving more farms and culturing of A. marginale would provide stronger statistical power to define acaricide usage association with OTC resistance as well as assessing invitro anti-microbial resistance profiles.