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Mopti and savanna molecular forms of the malaria vector anopheles Gambiae S.S populations from Bugiri and Iganga south–eastern Uganda

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dc.contributor.author Namusoke, Mariam
dc.date.accessioned 2017-04-11T02:33:12Z
dc.date.available 2017-04-11T02:33:12Z
dc.date.issued 2014-05
dc.identifier.citation Namusoke, M. (2014). Mopti and savanna molecular forms of the malaria vector anopheles Gambiae S.S populations from Bugiri and Iganga south–eastern Uganda. Master's thesis, Makerere University, Kampala, Uganda. en_US
dc.identifier.uri http://hdl.handle.net/10570/5572
dc.description A dissertation submitted to Directorate of Research and Graduate Training Makerere University in partial fulfilment for the award of the Degree of Master of Science in Molecular Biology and Biotechnology en_US
dc.description.abstract Female anopheles mosquitoes transmit malaria in humans and other mammals; Anopheles gambiae sensu stricto which is the primary vector of Plasmodium falciparum has been classified into two molecular forms, Savanna and Mopti. It had previously been suggested that there exist differences between these two forms in both insecticide resistance traits and susceptibility to P. falciparum. This study aimed at establishing the distribution of malaria vector species and molecular forms of An. gambiae s.s populations in Iganga and Bugiri districts south-eastern Uganda. Such entomological studies contribute to knowledge required by vector control programs and implementation of other public health intervention programs such as the use of indoor Residue spraying and insecticide treated nets. To this end, mosquitoes were collected between November and December 2010 using indoor pyrethrum spray collection from two villages of both Iganga and Bugiri districts. Morphological identification of species was done as previously described and mosquito species and molecular forms of An. gambiae s.s were done using PCR. A total of 665 samples were analysed. Out of 284 mosquitoes collected from Bugiri, 222 samples (78.1%) were An. arabiensis, 43 (15.1%) An. funestus, 12(4.2%) An. gambiae s.s and less than 5% unidentified. In Iganga, of the 381 mosquitoes identified with PCR, 200 (52.5%) were An. gambiae s.s, 123(32.2%) An. funestus, 49(12.9%) were An. arabiensis and the rest were unidentified. A total of 212 An. gambiae s.s identified were characterised further into molecular forms and all were identified as savanna molecular form. No Mopti or hybrid patterns of An. gambiae s.s were detected. Savanna molecular form of An. gambiae s.s is the only molecular form found in South Eastern Uganda. More extensive studies should be done to detect new evolving species in Uganda and other molecular identification assays developed using different sites apart from rDNA. Female anopheles mosquitoes transmit malaria in humans and other mammals; Anopheles gambiae sensu stricto which is the primary vector of Plasmodium falciparum has been classified into two molecular forms, Savanna and Mopti. It had previously been suggested that there exist differences between these two forms in both insecticide resistance traits and susceptibility to P. falciparum. This study aimed at establishing the distribution of malaria vector species and molecular forms of An. gambiae s.s populations in Iganga and Bugiri districts south-eastern Uganda. Such entomological studies contribute to knowledge required by vector control programs and implementation of other public health intervention programs such as the use of indoor Residue spraying and insecticide treated nets. To this end, mosquitoes were collected between November and December 2010 using indoor pyrethrum spray collection from two villages of both Iganga and Bugiri districts. Morphological identification of species was done as previously described and mosquito species and molecular forms of An. gambiae s.s were done using PCR. A total of 665 samples were analysed. Out of 284 mosquitoes collected from Bugiri, 222 samples (78.1%) were An. arabiensis, 43 (15.1%) An. funestus, 12(4.2%) An. gambiae s.s and less than 5% unidentified. In Iganga, of the 381 mosquitoes identified with PCR, 200 (52.5%) were An. gambiae s.s, 123(32.2%) An. funestus, 49(12.9%) were An. arabiensis and the rest were unidentified. A total of 212 An. gambiae s.s identified were characterised further into molecular forms and all were identified as savanna molecular form. No Mopti or hybrid patterns of An. gambiae s.s were detected. Savanna molecular form of An. gambiae s.s is the only molecular form found in South Eastern Uganda. More extensive studies should be done to detect new evolving species in Uganda and other molecular identification assays developed using different sites apart from rDNA. en_US
dc.description.sponsorship Trans Malaria Block (Transmalbloc) and EVIMalar of FP7 network en_US
dc.language.iso en en_US
dc.publisher Makerere University en_US
dc.subject Plasmodium falciparum en_US
dc.subject Anopheles mosquitoes en_US
dc.subject Malaria vector, Uganda en_US
dc.subject Anopheles Gambiae Sensu Stricto en_US
dc.title Mopti and savanna molecular forms of the malaria vector anopheles Gambiae S.S populations from Bugiri and Iganga south–eastern Uganda en_US
dc.type Thesis/Dissertation (Masters) en_US


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