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|Title: ||Molecular characterization of mycobacterium bovis isolates from cattle carcases at a city slaughterhouse in Uganda|
|Authors: ||Asiimwe, B. B.|
Ashaba, F. K.
|Keywords: ||Mycobacterium bovis|
|Issue Date: ||23-May-2009 |
|Publisher: ||British Veterinary Association|
|Citation: ||The Veterinary Record (2009) 164, 655-658|
|Abstract: ||During a period of eight months, the carcases of 16,800 slaughter cattle were inspected at a city abattoir in Uganda. Eighty-seven of them had tuberculosis-like lesions and tissue samples were cultured. Only 17 cultures yielded acid-fast bacilli; 11 of them were confirmed as Mycobacterium bovis and six as non-tuberculous mycobacteria (NTM).
GenoType Mycobacterium assays on the six NTM
identified two as Mycobacterium fortuitum and one as Mycobacterium intracellulare, but three were unidentified.
Characterisation of the M bovis isolates by spoligotyping and 156110 restriction fragment length polymorphism (RFLP) revealed that five of the six spoligotype patterns observed in the 11 strains had
not been previously reported, and seven of the nine isolates typed by RFLP had multicopy number 156110 patterns. Six of the 11 infected carcases had multiple sites of infection, but none was condemned as unfit for human consumption.|
|Description: ||This is a pdf scan of the author's hard print copy from the publisher (the Veterinary Record).|
|Appears in Collections:||Research Articles (Bio-Medical)|
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