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|Title: ||Serological and virological investigations on an emerging Peste des Petits Ruminants Virus infection in sheep and goats in Tanzania|
|Authors: ||Kivaria, F. M.|
Kapaga, A. M.
Mpelumbe-Ngeleja, C. A. R.
Tinuga, D. K.
|Keywords: ||Competitive enzyme linked immunosorbent assay (ELISA),|
Peste des petitruminants (PPR),
Real time PCR,
|Issue Date: ||Nov-2009 |
|Citation: ||The 13th East, Central and Southern African Commonwealth Veterinary Association Regional Meeting and International Scientific Conference, Kampala, Uganda, 8-13 November 2009|
|Abstract: ||Peste des petits ruminants virus (PPRV, genus Morbillivirus), which causes a severe disease in sheep and goats, has only recently been officially declared to be present in Tanzania. An exploratory cross-section study was carried out in August 2008 to determine the sero-prevalence, distribution, isolate and characterize an emerging PPRV infection in sheep and goats in Tanzania.
A multistage sampling strategy with 4 hierarchical stages was used to investigate sero-prevalence PPR status, and risk indicator for positive serological status in agro-pastoral and pastoral livestock farming systems in 7 districts of northern Tanzania. A total of 1546 serum samples from small ruminants reared in 48 villages from the 7 districts, were investigated; virologic and molecular epidemiologic techniques were applied to isolate, characterize and trace the origin of the PPRV in Tanzania. Prevalence for PPRV infection varied (range 0.00%–13.99%) and was higher in goats (50%) than in sheep (40%). The overall antibody response to PPRV was 45.00% (CI95% 43-47%); the proportion of sero-positive animals significantly (P = 0.05) differed between species, age groups, sex categories and farming practices. PPRV was isolated from sheep blood and tissues samples, real time PCR results showed that the isolated strains belong to lineage III whose origin is in the East Africa and the Middle East; thus one of the neighboring countries in the Eastern Africa region is most likely the source of infection.|
|Appears in Collections:||Conference and Workshop Reports (Vet)|
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