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Please use this identifier to cite or link to this item:
http://hdl.handle.net/123456789/477
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| Title: | Introduction of an in-house PCR for routine identification of M. tuberculosis in a low-income country |
| Authors: | Muhumuza, J.
Asiimwe, B. B.
Kayes, S.
Mugyenyi, P.
Whalen, C.
Mugerwa, R. D.
Boom, H.
Eisenach, K. D.
Joloba, M. L. |
| Keywords: | Mycobacterium tuberculosis
TB Identification
PCR
Uganda |
| Issue Date: | 7-Jul-2006 |
| Publisher: | International Union Against Tuberculosis and Lung Disease |
| Citation: | International Journal of Tuberculosis and Lung Disease, 2006, 10(11):1262–1267 |
| Abstract: | SETTING: National Tuberculosis (TB) Treatment Centre, Makerere University Medical School and Joint Clinical Research Centre, Kampala, Uganda.
OBJECTIVE: To evaluate the introduction of a polymerase chain reaction (PCR) based assay for identification of the Mycobacterium tuberculosis complex (MTC) into routine practice.
DESIGN: Routine diagnostic specimens were processed and inoculated into Bactec® 12B vials and monitored daily. At a growth index (GI) 10, 0.5 ml of the 12B broth was removed and assayed with PCR. The same
12B vial was analyzed using the Bactec® NAP method at GI 500. Vials at various levels of GI were included. Recurrent cost and time required to perform PCR and NAP were compared.
RESULTS: Initially, 71 specimens were analyzed; of these, 68 were NAP-positive while 69 were PCR-positive for MTC. PCR resulted in a 75% reduction in cost for a single test compared with Bactec® NAP. PCR has been successfully incorporated into routine practice, and 432 samples have been analyzed. In addition, isolates from solid
media were also well identified by PCR. With PCR, more samples can be analyzed at a time, it is faster and is less labor intensive.
CONCLUSION: PCR is a reliable and cheaper alternative for the identification of MTC. |
| URI: | http://www.ingentaconnect.com/content/iuatld/ijtld
http://hdl.handle.net/123456789/477 |
| ISSN: | 1027-3719 |
| Appears in Collections: | Research Articles (Bio-Medical)
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| muhumuza-asiimwe-joloba-chs-res.pdf | | 351Kb | Adobe PDF | View/Open |
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