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Please use this identifier to cite or link to this item:
http://hdl.handle.net/123456789/1832
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| Title: | Feasibility of magnetic bead technology for concentration of mycobacteria in sputum prior to fluorescence microscopy |
| Authors: | Albert, Heidi
Ademun, Patrick J.
Lukyamuzi, George
Nyesiga, Barnabas
Manabe, Yukari
Joloba, Moses L.
Wilson, Stuart
Perkins, Mark D |
| Keywords: | TB
Tuberclosis
Lung diseases
Mycobacterium tuberculosis
HIV/IDS
Smear preparation
Fluorescence microscopy |
| Issue Date: | May-2011 |
| Publisher: | BioMed Central |
| Citation: | Albert, H., Ademun, P.J., Lukyamuzi, G., Nyesiga, B., Manabe, Y., Joloba, M.L., Wilson, S., Perkins, M.D. (2011). Feasibility of magnetic bead technology for concentration of mycobacteria in sputum prior to fluorescence microscopy. BMC Infectious Diseases, 11(125) |
| Abstract: | Background: Direct sputum smear microscopy is the mainstay of TB diagnosis in most low and middle income
countries, and is highly specific for Mycobacterium tuberculosis in such settings. However it is limited by low
sensitivity, particularly in HIV co-infected patients. Concentration by centrifugation has been reported to be more
sensitive than direct smear preparation, but is only suitable for referral laboratories. Simpler concentration methods
that could be applied in peripheral laboratories are urgently needed.
Methods: We evaluated the feasibility of an early prototype ligand-coated magnetic bead technology to
concentrate M. tuberculosis prior to detection by LED-based fluorescence microscopy compared with direct Ziehl-
Neelsen microscopy and direct and concentrated fluorescence microscopy in a reference laboratory in Kampala,
Uganda. Results were compared with MGIT 960 liquid culture and Lowenstein-Jensen culture.
Results: Compared to culture, concentrated FM had significantly higher sensitivity than direct ZN (74.8% and 51.4%),
magnetic bead-FM (65.4%) and direct FM (58.9%). The sensitivity of magnetic bead FM was significantly higher than
direct ZN (p < 0.001) but not significantly higher than direct FM (p = 0.210). The specificity of magnetic bead FM and
concentrated FM was significantly lower than direct ZN (88.6%, 94.3% and 98.9% respectively) and direct FM (99.4%).
There was no significant difference in specificity between magnetic bead FM and concentrated FM. Allowing for
blinded resolution of discrepant results, the specificity of magnetic bead FM increased to 93.1%. Direct microscopy
was simpler than concentrated FM and Magnetic bead FM which both had a similar number of steps.
Conclusion: The sensitivity of the early prototype magnetic bead FM was lower than concentrated FM, similar to
direct FM, and significantly higher than direct ZN. Both magnetic bead and concentration by centrifugation led to
reduced specificity compared with the direct smear methods. Some magnetic bead FM false positive results were
not easily explained and should be further investigated. The prototype version of the magnetic bead procedure
tested here was of similar complexity to concentration by centrifugation. As such, if the sensitivity of the magnetic
bead FM could be improved in future versions of the technology, this may offer a viable alternative to centrifugation. |
| URI: | http://www.biomedcentral.com/1471-2334/11/125
doi:10.1186/1471-2334-11-125
http://hdl.handle.net/123456789/1832 |
| ISSN: | 1471-2334 |
| Appears in Collections: | Research Articles (Bio-Medical)
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