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Makerere University Research Repository >
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Please use this identifier to cite or link to this item:
http://hdl.handle.net/123456789/1761
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| Title: | Determination of Lamivudine, Zidovudine, and Nevirapine in capillary blood sampled on filter paper by LC. |
| Authors: | Obua, C
Malm, M.
Römsing, S.
Bergqvist, Y. |
| Keywords: | Antiretroviral therapy
HIV/AIDS
Sub-Saharan Africa |
| Issue Date: | 2009 |
| Publisher: | Preston Publications Inc |
| Citation: | Obua, C., Malm, M., Römsing, S., Bergqvist, Y. (2009). Determination of Lamivudine, Zidovudine, and Nevirapine in capillary blood sampled on filter paper by LC. Journal of Chromatographic Science, 47 |
| Abstract: | A bioanalytical method for determination of lamivudine (3TC), zidovudine (AZT), and nevirapine (NVP) in 100 μL capillary blood
applied onto sampling paper has been developed and validated. The
antiretroviral drugs (ARV) were analyzed by reversed phase
gradient liquid chromatography with UV detection. Separation was
performed on a Zorbax SB C8 (250 X 4.6 mm) column with a two step
gradient: (i) methanol–0.05 mol/L acetic acid-sodium acetate
buffer (pH 3.95, 15:85 v/v) and (ii) methanol–0.05 mol/L acetic
acid-sodium acetate buffer (pH 3.95, 50:50 v/v) with a flow rate of
1.0 mL/min. UV detection was performed at 260 nm. Total assay
precisions were 6.3, 4.7, and 4.9% for 3TC at 0.34, 0.69, and 3.9
μg/mL, and 5.1, 5.5, and 3.2% for AZT at 0.40, 0.80, and 4.5
μg/mL. For NVP, total assay precisions were 5.2, 8.3, and 3.5% at
2.6, 4.5, and 8.8 μg/mL. Lower limit of quantifications (LLOQ)
were 0.11 and 0.13 μg/mL for 3TC and AZT where the precisions were 2.0% for both the analytes. For NVP, LLOQ was 1.3 μg/mL where precision was 2.6%. Concentrations were determined for 10h for two subjects receiving standard twice daily antiretroviral therapy containing 3TC, AZT, and NVP. Maximum 3TC concentrations were 2.5 and 2.8 μg/mL for subject 1 and 2, respectively. For AZT, maximum concentrations were 1.8 and 1.1
μg/mL while being 15 and 9.6 μg/mL for NVP. Pre-dose trough concentration of NVP was 11 μg/mL for subject 1 and 9.6 μg/mL
for subject 2. |
| URI: | http://hdl.handle.net/123456789/1761 |
| ISSN: | 0021-9665 |
| Appears in Collections: | Research Articles (Health-Sciences)
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| obua-chs-res.pdf | | 367Kb | Adobe PDF | View/Open |
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