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Please use this identifier to cite or link to this item:
http://hdl.handle.net/123456789/1638
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| Title: | Monoclonal antibodies against enterocytozoon bieneusi of human origin |
| Authors: | Sheoran, Abhineet S.
Feng, Xiaochuan
Singh, Inderpal
Chapman-Bonofiglio, Susan
Kitaka, Sabrina
Hanawalt, Joel
Nunnari, John
Mansfield, Keith
Tumwine, James K.
Tzipori, Saul |
| Keywords: | Monoclonal antibodies
Enterocytozoon bieneusi
Enteric protozoon
E. bieneusi
Microsporidia |
| Issue Date: | 2005 |
| Publisher: | American Society for Microbiology |
| Citation: | Sheoran, A. S., et al. (2005). Monoclonal antibodies against Enterocytozoon bieneusi of
human origin. Clinical and Diagnostic Laboratory Immunology, 12(9):1109-1113 |
| Series/Report no.: | Clinical and Diagnostic Laboratory Immunology
12(9) |
| Abstract: | Enterocytozoon bieneusi is clinically the most significant among the microsporidia infecting humans, causing
chronic diarrhea, wasting, and cholangitis in individuals with human immunodeficiency virus/AIDS. The lack
of immune reagents is largely due to the absence of methods for laboratory propagation of E. bieneusi. We
recently described a procedure for the concentration and purification of spores from diarrheic stool of infected
humans. Purified spores were used to immunize mice for production and screening of monoclonal antibodies
(MAbs) against E. bieneusi. The eight immunoglobulin M MAbs generated and fully characterized did not
cross-react with other human microsporidia or with other microorganisms normally present in stool. One of
the MAbs, 2G4, reacted with E. bieneusi spores in stools from monkeys and humans, without background
fluorescence, which makes it an ideal diagnostic reagent. It also recognizes intracellular stages of the parasite
and will be suitable for determining tissue distribution of E. bieneusi in infected hosts. At least two immunodominant
antigens of E. bieneusi of 33,000 and 35,000 Da exist, which were recognized by rabbit and mouse
antisera. The availability of MAbs against E. bieneusi will simplify considerably the diagnosis of this infection
in humans and will provide tools for epidemiologic investigations regarding the true prevalence of the infection
in various human and mammalian populations and the environmental sources of infection |
| URI: | http://dx.doi.org/10.1128/CDLI.12.9.1109-1113.2005
http://hdl.handle.net/123456789/1638 |
| ISSN: | 1071-412X |
| Appears in Collections: | Research Articles (Sch. of Med.)
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