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|Title: ||Performance of two commercial immunochromatographic assays for rapid detection of antibodies specific to human immunodeficieny virus types 1 and 2 in serum and urine samples in a rural community-based research setting (Rakai, Uganda)|
|Authors: ||Kagulire, S. C.|
Stamper, P. D.
Nakavuma, J. L.
Mills, L. A.
Gray, R. H.
Reynolds, S. J.
Community based research
|Issue Date: ||Jun-2007 |
|Publisher: ||American Society for Microbiology|
|Citation: ||Kagulire, S.C., Stamper, P.D., Opendi, P., Nakavuma, J.L., Mills, L.A., Makumbi, F., Gray, R.H., Serwadda, D., Reynolds, S.J. (2007). Performance of two commercial immunochromatographic assays for rapid detection of antibodies specific to human immunodeficiency virus types 1 and 2 in serum and urine samples in a rural community-based research setting (Rakai, Uganda). Clinical and Vaccine Immunology, 14(6)|
|Abstract: ||Rapid detection of human immunodeficiency virus (HIV) antibodies is of great importance in developing and
developed countries to diagnose HIV infections quickly and at low cost. In this study, two new immunochromatographic
rapid tests for the detection of HIV antibodies (Aware HIV-1/2 BSP and Aware HIV-1/2 U; Calypte
Biomedical Corporation) were evaluated in rural Africa to determine the tests’ performance and comparability
to commercially available conventional enzyme immunoassay (EIA) and Western blot (WB) tests. This prospective
study was conducted from March 2005 through May 2005 using serum and urine from respondents in
the Rakai Community Cohort Survey. Nine hundred sixty-three serum samples were tested with the Aware
blood rapid assay (Aware-BSP) and compared to two independent EIAs for HIV plus confirmatory Calypte WB
for any positive EIAs. The sensitivity of Aware-BSP was 98.2%, and the specificity was 99.8%. Nine hundred
forty-two urine samples were run using the Aware urine assay (Aware-U) and linked to blood sample results
for analysis. The sensitivity of Aware-U was 88.7% and specificity was 99.9% compared to blood EIAs confirmed
by WB analysis. These results support the adoption of the Aware-BSP rapid test as an alternative to EIA and
WB assays for the diagnosis of HIV in resource-limited settings. However, the low sensitivity of the Aware-U
assay with its potential for falsely negative HIV results makes the urine assay less satisfactory.|
|Appears in Collections:||Research Articles (Vet)|
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