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|Title: ||A Combined CXCL10, CXCL8 and H-FABP Panel for the Staging of Human African Trypanosomiasis Patients|
|Authors: ||Hainard, Alexandre|
Ngoyi, Dieudonne´ Mumba
Enyaru, John Charles
Ndungu, Joseph Mathu
Human African Trypanosomiasis
|Issue Date: ||2009 |
|Publisher: ||Public Library of Science|
|Citation: ||Hainard, A., Tiberti, N., Robin, X., Lejon, V., Ngoyi, D.M., Matovu, E., Enyaru, J.C., Fouda, C., Ndungu, J.M., Lisacek, F., Muller, M., Turck, N. (2009). A combined CXCL10, CXCL8 and H-FABP panel for the staging of human African trypanosomiasis patients. PLoS Neglected Tropical Diseases, 3(6)|
|Abstract: ||Background: Human African trypanosomiasis (HAT), also known as sleeping sickness, is a parasitic tropical disease. It
progresses from the first, haemolymphatic stage to a neurological second stage due to invasion of parasites into the central
nervous system (CNS). As treatment depends on the stage of disease, there is a critical need for tools that efficiently
discriminate the two stages of HAT. We hypothesized that markers of brain damage discovered by proteomic strategies and
inflammation-related proteins could individually or in combination indicate the CNS invasion by the parasite.
Methods: Cerebrospinal fluid (CSF) originated from parasitologically confirmed Trypanosoma brucei gambiense patients.
Patients were staged on the basis of CSF white blood cell (WBC) count and presence of parasites in CSF. One hundred
samples were analysed: 21 from stage 1 (no trypanosomes in CSF and #5 WBC/mL) and 79 from stage 2 (trypanosomes in
CSF and/or .5 WBC/mL) patients. The concentration of H-FABP, GSTP-1 and S100b in CSF was measured by ELISA. The levels
of thirteen inflammation-related proteins (IL-1ra, IL-1b, IL-6, IL-9, IL-10, G-CSF, VEGF, IFN-c, TNF-a, CCL2, CCL4, CXCL8 and
CXCL10) were determined by bead suspension arrays.
Results: CXCL10 most accurately distinguished stage 1 and stage 2 patients, with a sensitivity of 84% and specificity of
100%. Rule Induction Like (RIL) analysis defined a panel characterized by CXCL10, CXCL8 and H-FABP that improved the
detection of stage 2 patients to 97% sensitivity and 100% specificity.
Conclusion: This study highlights the value of CXCL10 as a single biomarker for staging T. b. gambiense-infected HAT
patients. Further combination of CXCL10 with H-FABP and CXCL8 results in a panel that efficiently rules in stage 2 HAT
patients. As these molecules could potentially be markers of other CNS infections and disorders, these results should be
validated in a larger multi-centric cohort including other inflammatory diseases such as cerebral malaria and active
|Appears in Collections:||Research Articles (Vet)|
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