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Please use this identifier to cite or link to this item:
http://hdl.handle.net/123456789/1476
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| Title: | A model for mapping of Ebola and Marburg RNA integration sites in rhesus Macaca mulatta genome in silico: Ebola virus acceptors sites located on chromosones 4, 6, 7, 8, 9, 14 and 15 |
| Authors: | Wayengera, Misaki
Byarugaba, Wilson
Kajjumbula, Henry
Olobo, J.
Mulindwa, Kaddu |
| Keywords: | Ebola
Marburg
In-vivo integration
Rhesus macaca
line elements
Insilico genomics |
| Issue Date: | 2009 |
| Publisher: | Academic Journals |
| Citation: | Wayengera, M., Kajumbula, H., Mulindwa, K.D., Olobo, J., Byarugaba, W. (2009). A model for mapping of Ebola and Marburg RNA integration sites in rhesus Macaca mulatta genome in silico: Ebola virus acceptors sites located on chromosomes 4, 6, 7, 8, 9, 14 and 15. African Journal of Biotechnology, 8 (10) |
| Abstract: | Viral integration into the host genetic material is necessary for replication and survival, since viruses
are obligate intracellular organisms. Understanding of the exact loci of integration may thus provide
targets for future therapeutic and vaccine strategies, pathogenesis elucidation, as well as a model for
the evolutionary trends of successful viral cross over. Although the exact natural reservoir for the
filovirade family of viruses still remains elusive, most index cases in human outbreaks have been linked
to contact with nonhuman primates (NHP). We hypothesized that homogeneity between viral integration
complex and host genome may be a major predictor of integration. To investigate and map the loci of
integration of the two major genes of this family of viruses within NHP genomes, we queried both Ebola
and Marburg Glycoprotein (GP) gene sequences against the whole genome of rhesus macaque using
BLAST-N analysis. Of all the contigs length 2.87 Gb (2,863,665,185) bases in the genome of rhesus
macaque, Marburg GP blast hits to rhesus genome nucleotide database were 6,451,736 compared to
4,012,901 for Ebola. Marburg GP genomic RNA had 18 alignments located on undefined scaffolds
compared to 7 of Ebola located on chromosomes 4, 6, 7, 8, 9, 14 and 15. We also found an efficiency of
66.6% within Marburg GP alignments compared to 100% for Ebola. Our results serve to demonstrate
that although Marburg GP RNA acceptors are more prevalent in the Rhesus genome than ebola; their
loci of integration are vaguely defined compared to Ebola. If the level of homogeneity between
acceptors and PIC has no effect of integration, then Marburg may be better adapted to integrate into
Rhesus that Ebola. Alternatively, chromatic DNA might be a more effective target for future Ebola
genomic vaccines sequestered at a nuclear location inaccessible to incoming Pre-integration
Complexes (PICs-which in this model are Ebola glycoprotein gene complexes) than Marburg. |
| URI: | http://hdl.handle.net/123456789/1476 |
| ISSN: | 1684–5315 |
| Appears in Collections: | Research Articles (Health-Sciences)
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| Wayengera-chs-res.pdf | | 81Kb | Adobe PDF | View/Open |
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