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Please use this identifier to cite or link to this item:
http://hdl.handle.net/123456789/1475
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| Title: | Proteomics of Marburg and Ebola glycoproteins: Insights into their physiocochemical similarities and irregularities |
| Authors: | Wayengera, Misaki
Kajumbula, Henry
Mulindwa, Kaddu D.
Olobo, Joseph
Byarugaba, Wilson |
| Keywords: | Ebola
Marburg
Glycoproteins
Proteomics
Vaccine research
Infectious diseases
Viral hemorrhagic fevers (VHF) |
| Issue Date: | 2009 |
| Publisher: | Academic Journals |
| Citation: | Wayengera, M., Kajumbula, H., Mulindwa, K.D., Olobo, J., Byarugaba, W. (2009). Proteomics of Marburg and Ebola glycoproteins: insights into their physicochemical similarities and irregularities. African Journal of Biotechnology, 8 (17) |
| Abstract: | Ebola (EBO) and Marburg (MBG) form the two major genus of a class of negatively stranded RNA
viruses responsible for viral hemorrhagic fevers (VHF) in equatorial Africa called filoviridae.
Pathogenesis of VHF is thought to be mediated by the envelope glycoproteins (GP). Although both
viruses have been found to be closely related, with a side by side alignment of the gene sequences of
the Ebola and Marburg viruses and comparison of protein products showing similarities, there is
absence of immunological cross reactivity. The physicochemical properties of the two GP may in part
explain this. Using a 676 aa Ebola and 681 aa Marburg GP protein sequences; and the ExPASy Server
ProtParam, the physicochemical properties of both proteins were derived and compared. The MBG-GP
molecular weight was bigger and more negatively charged than EBO (MW=74481 vs.74464.4 and overall
charge=-11 vs.-9). Other constants analyzed respectively: for MBG versus EBO (1) theoretical PI: 5.85
vs. 6.16, (2) extinction coefficient (82110/1.102 vs 100100/1.344 M-1 cm-1 at 280 nm/H2O). The Estimated
half life for both glycoproteins was 30 h, although MBG-GP was unstable compared to EBO-GP (The
instability index [II]; MBG vs EBO = 42.82 vs. 38.36). These variations must be taken into account in
research and development of a common vaccine against both MBG and EBO. |
| URI: | http://www.academicjournals.org/AJB
http://hdl.handle.net/123456789/1475 |
| ISSN: | 1684–5315 |
| Appears in Collections: | Research Articles (Health-Sciences)
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| Wayengera-chs-res.pdf | | 84Kb | Adobe PDF | View/Open |
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