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Makerere University Research Repository >
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Please use this identifier to cite or link to this item:
http://hdl.handle.net/123456789/1429
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| Title: | Evaluation of Dynabeads and Cytospheres Compared With Flow Cytometry to Enumerate CD41 T Cells in HIV-Infected Ugandans on Antiretroviral Therapy |
| Authors: | Lutwama, Fred
Serwadda, Ronnie
Mayanja-Kizza, Harriet
Shihab, Hasan M
Ronald, Allan
Kamya, Moses R
Thomas, David
Johnson, Elizabeth
Quinn, Thomas C
Moore, Richard D
Spacek, Lisa A. |
| Keywords: | CD4 lymphocyte counts
HIV-1 viral load
Monitoring and evaluation
Antiretroviral therapy
Resource-limited setting
Uganda |
| Issue Date: | 1-Jul-2008 |
| Publisher: | Lippincott Williams & Wilkins. |
| Citation: | Lutwama F,Serwadda R,Mayanja-Kizza H,Shihab H,Ronald A,Kamya MR,Thomas D,Johnson E,Quinn T, Moore R,Spacek L.Evaluation of Dynabeads and Cytospheres Compared With Flow Cytometry to Enumerate CD41 T Cells in HIV-Infected Ugandans on Antiretroviral Therapy.Vol 48, N0_3. |
| Abstract: | Background: Laboratory-based monitoring of antiretroviral therapy
is essential but adds a significant cost to HIV care. The World Health
Organization 2006 guidelines support the use of CD4 lymphocyte
count (CD4) to define treatment failure in resource-limited settings.
Methods: We compared CD4 obtained on replicate samples from
497 HIV-positive Ugandans (before and during ART) followed for
18 months by 2 manual bead–based assays, Dynabeads (Dynal
Biotech), and Cytospheres (Beckman Coulter) with those generated by
flow cytometry at the Infectious Diseases Institute in Kampala, Uganda.
Results: We tested 1671 samples (123 before ART) with Dynabeads
and 1444 samples (91 before ART) with Cytospheres. Mean CD4 was
231 cells/mm3 (SD, 139) and 239 cells/mm3 (SD, 140) by Dynabeads
and flow cytometry, respectively. Mean CD4 was 186 cells/mm3 (SD,
101) and 242 cells/mm3 (SD, 136) by Cytospheres and flow cytometry,
respectively. The mean difference in CD4 count by flow cytometry
versus Dynabeads were 8.8 cells/mm3 (SD, 76.0) and versus Cytospheres
were 56.8 cells/mm3 (SD, 85.8). The limits of agreement
were 2140.9 to 158.4 cells/mm3 for Dynabeads and 2112.2 to
225.8 cells/mm3 for Cytospheres. Linear regression analysis showed
higher correlation between flow cytometry and Dynabeads (r = 0.85,
r2 = 0.73, slope = 0.85, intercept = 28) compared with the correlation
between flow cytometry and Cytospheres (r = 0.78, r2 = 0.60, slope =
0.58, intercept = 45). Area under the receiver operating characteristics
curve to predict CD4 ,200 cells/mm3 was 0.928 for Dynabeads and
0.886 for Cytospheres.
Conclusion: Although Dynabeads and Cytospheres both underestimated
CD4 lymphocyte count compared with flow cytometry, in
resource-limited settings with low daily throughput, manual bead–
based assays may provide a less expensive alternative to flow cytometry. |
| URI: | http://hdl.handle.net/123456789/1429 |
| Appears in Collections: | Research Articles (IDI)
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| Lutwama fred-idi-res1.pdf | | 181Kb | Adobe PDF | View/Open |
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