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|Title: ||Mycobacterium africanum subtype II is associated with two distinct genotypes and is a major cause of human tuberculosis in Kampala, Uganda|
|Authors: ||Niemann, S.|
Joloba, M. L.
Whalen, C. C.
Ellner, J. J.
Johnson, J. L.
Mugerwa, R. D.
Schwander, S. K.
|Keywords: ||Mycobacterium tuberculosis|
Mycobacterium africanum Subtype II
|Issue Date: ||Sep-2002 |
|Publisher: ||American Society for Microbiology|
|Citation: ||Niemann, S., Rusch-Gerdes, S., Joloba, M. L., Whalen, C. C., Guwatudde, D., Ellner, J.J., Eisenach, K., Fumokong, N., Johnson, J.L., Aisu, T., Mugerwa, R.D., Okwera, A., Schwander, S. K. (2002). Mycobacterium africanum subtype II is associated with two distinct genotypes and is a major cause of human tuberculosis in Kampala, Uganda. Journal of Clinical Microbiology, 40(9).|
|Abstract: ||The population structure of 234 Mycobacterium tuberculosis complex strains obtained during 1995 and 1997
from tuberculosis patients living in Kampala, Uganda (East Africa), was analyzed by routine laboratory
procedures, spoligotyping, and IS6110 restriction fragment length polymorphism (RFLP) typing. According to
biochemical test results, 157 isolates (67%) were classified as M. africanum subtype II (resistant to thiophen-
2-carboxylic acid hydrazide), 76 isolates (32%) were classified as M. tuberculosis, and 1 isolate was classified as
classical M. bovis. Spoligotyping did not lead to clear differentiation of M. tuberculosis and M. africanum, but
all M. africanum subtype II isolates lacked spacers 33 to 36, differentiating them from M. africanum subtype I.
Moreover, spoligotyping was not sufficient for differentiation of isolates on the strain level, since 193 (82%)
were grouped into clusters. In contrast, in the IS6110-based dendrogram, M. africanum strains were clustered
into two closely related strain families (Uganda I and II) and clearly separated from the M. tuberculosis isolates.
A further characteristic of both M. africanum subtype II families was the absence of spoligotype spacer 40. All
strains of family I also lacked spacer 43. The clustering rate obtained by the combination of spoligotyping and
RFLP IS6110 analysis was similar for M. africanum and M. tuberculosis, as 46% and 49% of the respective
isolates were grouped into clusters. The results presented demonstrate that M. africanum subtype II isolates
from Kampala, Uganda, belong to two closely related genotypes, which may represent unique phylogenetic
branches within the M. tuberculosis complex. We conclude that M. africanum subtype II is the main cause of
human tuberculosis in Kampala, Uganda.|
|Appears in Collections:||Research Articles (Bio-Medical)|
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