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|Title: ||The use of Genoype MTBDRplus assay to assess drug resistance of microbacterium tuberculosis isolates from patients in rural Uganda|
|Authors: ||Bazira, Joel|
Asiimwe, Benon B.
Joloba, Moses L.
Matee, Mecky I.
|Keywords: ||Mycobacterium tuberculosis|
Multidrug Resistant-Tuberclosis (MDR-TB)
|Issue Date: ||2010 |
|Publisher: ||BioMed Central|
|Citation: ||Bazira, J., Asiimwe, B.B., Joloba, M.L., Bwanga, F., Mecky I Matee, M.I. (2010). Use of the GenoType® MTBDRplus assay to assess drug resistance of Mycobacterium tuberculosis isolates from patients in rural Uganda. BMC Clinical Pathology 2010, 10(5)|
|Abstract: ||Background: Drug resistance levels and patterns among Mycobacterium tuberculosis isolates from newly diagnosed and previously treated tuberculosis patients in Mbarara Uganda were investigated.
Methods: We enrolled, consecutively; all newly diagnosed and previously treated smear-positive TB patients aged ≥ 18 years. Isolates were tested for drug resistance against rifampicin (RIF) and isoniazid (INH) using the Genotype® MDRTBplus assay and results were compared with those obtained by the indirect proportion method on Lowenstein-Jensen media. HIV testing was performed using two rapid HIV tests.
Results: A total of 125 isolates from 167 TB suspects with a mean age 33.7 years and HIV prevalence of 67.9% (55/81) were analysed. A majority (92.8%) of the participants were newly presenting while only 7.2% were retreatment cases. Resistance mutations to either RIF or INH were detected in 6.4% of the total isolates. Multidrug resistance, INH and RIF resistance was 1.6%, 3.2% and 4.8%, respectively. The rpob gene mutations seen in the sample were D516V, S531L, H526Y H526 D and D516V, while one strain had a Δ1 mutation in the wild type probes. There were three strains with katG (codon 315) gene mutations while only one strain showed the inhA promoter region gene mutation.
Conclusion: The TB resistance rate in Mbarara is relatively low. The GenoType® MTBDRplus assay can be used for rapid screening of MDR-TB in this setting.|
|Appears in Collections:||Research Articles (Health-Sciences)|
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