|
Makerere University Research Repository >
College of Health Sciences >
School of Health Sciences >
Research Articles (Health-Sciences) >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/123456789/1283
|
| Title: | The use of Genoype MTBDRplus assay to assess drug resistance of microbacterium tuberculosis isolates from patients in rural Uganda |
| Authors: | Bazira, Joel
Asiimwe, Benon B.
Joloba, Moses L.
Bwanga, Fred
Matee, Mecky I. |
| Keywords: | Mycobacterium tuberculosis
TB (Disease)
Pulmonary tuberclosis
Lungs-Diseases
Lungs-Tuberclosis
HIV/AIDS
Multidrug Resistant-Tuberclosis (MDR-TB)
Consumption (Disease) |
| Issue Date: | 2010 |
| Publisher: | BioMed Central |
| Citation: | Bazira, J., Asiimwe, B.B., Joloba, M.L., Bwanga, F., Mecky I Matee, M.I. (2010). Use of the GenoType® MTBDRplus assay to assess drug resistance of Mycobacterium tuberculosis isolates from patients in rural Uganda. BMC Clinical Pathology 2010, 10(5) |
| Abstract: | Background: Drug resistance levels and patterns among Mycobacterium tuberculosis isolates from newly diagnosed and previously treated tuberculosis patients in Mbarara Uganda were investigated.
Methods: We enrolled, consecutively; all newly diagnosed and previously treated smear-positive TB patients aged ≥ 18 years. Isolates were tested for drug resistance against rifampicin (RIF) and isoniazid (INH) using the Genotype® MDRTBplus assay and results were compared with those obtained by the indirect proportion method on Lowenstein-Jensen media. HIV testing was performed using two rapid HIV tests.
Results: A total of 125 isolates from 167 TB suspects with a mean age 33.7 years and HIV prevalence of 67.9% (55/81) were analysed. A majority (92.8%) of the participants were newly presenting while only 7.2% were retreatment cases. Resistance mutations to either RIF or INH were detected in 6.4% of the total isolates. Multidrug resistance, INH and RIF resistance was 1.6%, 3.2% and 4.8%, respectively. The rpob gene mutations seen in the sample were D516V, S531L, H526Y H526 D and D516V, while one strain had a Δ1 mutation in the wild type probes. There were three strains with katG (codon 315) gene mutations while only one strain showed the inhA promoter region gene mutation.
Conclusion: The TB resistance rate in Mbarara is relatively low. The GenoType® MTBDRplus assay can be used for rapid screening of MDR-TB in this setting. |
| URI: | http://hdl.handle.net/123456789/1283 |
| ISSN: | 1472-6890 |
| Appears in Collections: | Research Articles (Health-Sciences)
|
Files in This Item:
| File |
Description |
Size | Format |
|---|
| bazira-joel-chs-res.pdf | | 214Kb | Adobe PDF | View/Open |
|
All items in DSpace are protected by copyright, with all rights reserved.
|
